Confocal image of an immunostained sectioned blister. A. Nerves (protein gene product 9.5 immunoreactivity) are green or yellow, basement membrane (type IV collagen-immunoreactivity) is red. Epidermis separated from dermis just above the dermo-epidermal basement membrane. Scale bar = 200µm. B. Dermal capillaries and the subepidermal neural plexus remained intact. Scale bar = 100µm. C. Epidermal nerves, severed from their proximal segment, remained in the blister roof. Scale bar = 50µm.